ABSTRACT
Objective:To evaluate the effect of down-regulation of Nav1.7 on the activation of astrocytes and microglia in DRG of rats with cancer pain, and explore the transmission of the nociceptive information.Methods:Lentiviral vector harboring RNAi sequence targeting theNav1.7gene was constructed, and Walker 256 breast cancer cell and morphine was injected to build the bone cancer pain model and morphine tolerance model in rats. Lentiviral vector was injected. Rats in each model were divided into 4 groups: model group, PBS group, vehicle group and LV-Nav1.7 group. The expression levels of GFAP and OX42 in dorsal root ganglia (DRG) were measured.Results: After the animal model was built,the level of Nav1.7, GFAP and OX42 was improved obviously with the time prolonged, which was statistically significant (P<0.05). The expression level of GFAP and OX42 in the DRG in the LV-Nav1.7 group declined obviously compared to the model group, PBS group and vehicle group (P<0.05).Conclusions:Intrathecal injection of Navl.7 shRNA lentiviral vector can reduce the expression of Nav1.7 and inhibit the activation of astrocytes and microglia in DRG. The effort is also effective in morphine tolerance bone cancer pain model rats.
ABSTRACT
Objective To analyze the epidemiological features of hip fraction,and to improve the intervention program on this disease.Methods To investigate the clinical data of the patients with hip fraction who were treated at local hospitals,from Jan.to Dec.,2012.Information regarding sex,age,site and cause of the fracture was analyzed.Results 877 cases were treated at the local hospitals; including 516 males (58.84％) and 361 females (41.16％).The overall incidence of hip fraction was 10.0/100 000,with 11.2/100 000 in males and 8.8/100 000 in females.The incidence was higher in males than that in females (x2=4.281,P=0.033).Age distribution of the patients was:344 cases in age 71-82 (39.22％),196 cases in age 61-70 (22.35％) and 185 cases in age 51-60 (21.09％).Transcervical fracture appeared more than intertrochanter fracture of femur (x2=21.423,P＜0.001),with males more than females in both fractures on femur (x2=12.816,P＜0.001 ; x2=13.773,P＜0.001).The top 3 factors causing hip fractions would contain tumble (64.88％),falling (20.07％) and traffic accident (10.49％).Conclusion Incidence of hip fraction would increase with age with tumble as the major cause to it.